General 1,5-AG(1,5-Anhydroglucitol) ELISA Kit

Description

Catalogue Number:SPK7840
Name::General 1,5-AG(1,5-Anhydroglucitol) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 0.6 μg/mL
Standard: 150 μg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Enzyme & Kinase;Metabolic pathway;Endocrinology;Nutrition metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 1,5-Anhydroglucitol(1,5-AG) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 1,5-Anhydroglucitol(1,5-AG). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 1,5-Anhydroglucitol(1,5-AG) in the samples is then determined by comparing the OD of the samples to the standard curve.