General BA(Butyric Acid) ELISA Kit

Description

Catalogue Number:SPK8174
Name::General BA(Butyric Acid) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 51.5 pg/mL
Standard: 10000 pg/mL
Sample Type: Biological agents
Assay Length: 2h
Research Area: Signal transduction;Metabolic pathway;Endocrinology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Butyric Acid(BA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Butyric Acid(BA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Butyric Acid(BA) in the samples is then determined by comparing the OD of the samples to the standard curve.