General F-E3(Free Estriol) ELISA Kit

Description

Catalogue Number:SPK9646
Name::General F-E3(Free Estriol) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 0.218 ng/mL
Standard: 40 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Free Estriol(F-E3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Free Estriol(F-E3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Free Estriol(F-E3) in the samples is then determined by comparing the OD of the samples to the standard curve.