General VMA(Vanillylmandelic Acid) ELISA Kit

Description

Catalogue Number:SPK8239
Name::General VMA(Vanillylmandelic Acid) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 43.7 ng/mL
Standard: 10000 ng/mL
Sample Type: urine and biological agents
Assay Length: 2h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Gastroenterology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vanillylmandelic Acid(VMA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vanillylmandelic Acid(VMA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vanillylmandelic Acid(VMA) in the samples is then determined by comparing the OD of the samples to the standard curve.