Goat HB(Hemoglobin) ELISA Kit

Description

Catalogue Number:SPK4257
Name::Goat HB(Hemoglobin) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 2.7 ng/mL
Standard: 500 ng/mL
Sample Type: Serum, plasma, erythrocyte lysates.
Assay Length: 3.5h
Research Area: Metabolic pathway;Infection immunity;Hematology;Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin(HB). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin(HB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin(HB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hemoglobin(HB) in the samples is then determined by comparing the OD of the samples to the standard curve.