Horse DBP(Vitamin D Binding Protein) ELISA Kit

Description

Catalogue Number:SPK8281
Name::Horse DBP(Vitamin D Binding Protein) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 2.84 ng/mL
Standard: 400 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Metabolic pathway;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Binding Protein(DBP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin D Binding Protein(DBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Binding Protein(DBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D Binding Protein(DBP) in the samples is then determined by comparing the OD of the samples to the standard curve.