Human 20S-PSM(20S-Proteasome) ELISA Kit

Description

Catalogue Number:SPK5359
Name::Human 20S-PSM(20S-Proteasome) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.66 ng/mL
Standard: 100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 20S-Proteasome(20S-PSM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 20S-Proteasome(20S-PSM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 20S-Proteasome(20S-PSM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 20S-Proteasome(20S-PSM) in the samples is then determined by comparing the OD of the samples to the standard curve.