Human a1ACT(Alpha-1-Antichymotrypsin) ELISA Kit

Description

Catalogue Number:SPK1732
Name::Human a1ACT(Alpha-1-Antichymotrypsin) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 1.47 ng/mL
Standard: 200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Infection immunity;Neuro science;Pulmonology;Hepatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antichymotrypsin(a1ACT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Alpha-1-Antichymotrypsin(a1ACT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antichymotrypsin(a1ACT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antichymotrypsin(a1ACT) in the samples is then determined by comparing the OD of the samples to the standard curve.