Human Anti-CRP(Anti-C Reactive Protein Antibody) ELISA Kit

Description

Catalogue Number:SPK8060
Name::Human Anti-CRP(Anti-C Reactive Protein Antibody) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 1.14 ng/mL
Standard: 200 ng/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h, 30min
Research Area: Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Anti-C Reactive Protein Antibody(Anti-CRP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Anti-C Reactive Protein Antibody(Anti-CRP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-C Reactive Protein Antibody(Anti-CRP) in the samples is then determined by comparing the OD of the samples to the standard curve.