Human AP12(Apelin 12) ELISA Kit

Description

Catalogue Number:SPK1635
Name::Human AP12(Apelin 12) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 26.52 pg/mL
Standard: 6000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Gastroenterology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin 12(AP12) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin 12(AP12). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apelin 12(AP12) in the samples is then determined by comparing the OD of the samples to the standard curve.