Human AP13(Apelin 13) ELISA Kit

Description

Catalogue Number:SPK2252
Name::Human AP13(Apelin 13) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 8.24 pg/mL
Standard: 1600 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apelin 13(AP13) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apelin 13(AP13). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apelin 13(AP13) in the samples is then determined by comparing the OD of the samples to the standard curve.