Human APOL4(Apolipoprotein L4) ELISA Kit

Description

Catalogue Number:SPK8783
Name::Human APOL4(Apolipoprotein L4) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.062 ng/mL
Standard: 10 ng/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein L4(APOL4). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apolipoprotein L4(APOL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein L4(APOL4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein L4(APOL4) in the samples is then determined by comparing the OD of the samples to the standard curve.