Human ARNT(Aryl Hydrocarbon Receptor Nuclear Translocator) ELISA Kit

Description

Catalogue Number:SPK4776
Name::Human ARNT(Aryl Hydrocarbon Receptor Nuclear Translocator) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.058 ng/mL
Standard: 10 ng/mL
Sample Type: tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator(ARNT). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator(ARNT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor Nuclear Translocator(ARNT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aryl Hydrocarbon Receptor Nuclear Translocator(ARNT) in the samples is then determined by comparing the OD of the samples to the standard curve.