Human CD74(HLA class II histocompatibility antigen gamma chain) ELISA Kit

Description

Catalogue Number:SPK9139
Name::Human CD74(HLA class II histocompatibility antigen gamma chain) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.294 ng/mL
Standard: 40 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Immunology
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA class II histocompatibility antigen gamma chain(CD74). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HLA class II histocompatibility antigen gamma chain(CD74). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HLA class II histocompatibility antigen gamma chain(CD74), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HLA class II histocompatibility antigen gamma chain(CD74) in the samples is then determined by comparing the OD of the samples to the standard curve.