Human CLIP(Corticotropin Like Intermediate Lobe Peptide) ELISA Kit

Description

Catalogue Number:SPK4253
Name::Human CLIP(Corticotropin Like Intermediate Lobe Peptide) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 4.89 pg/mL
Standard: 1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Hormone metabolism;Urology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Corticotropin Like Intermediate Lobe Peptide(CLIP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticotropin Like Intermediate Lobe Peptide(CLIP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticotropin Like Intermediate Lobe Peptide(CLIP) in the samples is then determined by comparing the OD of the samples to the standard curve.