Human D2HGDH(D2-Hydroxyglutarate Dehydrogenase) ELISA Kit

Description

Catalogue Number:SPK5013
Name::Human D2HGDH(D2-Hydroxyglutarate Dehydrogenase) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.065 ng/mL
Standard: 10 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D2-Hydroxyglutarate Dehydrogenase(D2HGDH). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to D2-Hydroxyglutarate Dehydrogenase(D2HGDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D2-Hydroxyglutarate Dehydrogenase(D2HGDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D2-Hydroxyglutarate Dehydrogenase(D2HGDH) in the samples is then determined by comparing the OD of the samples to the standard curve.