Human dsDNA(anti-double stranded DNA antibody IgG) ELISA Kit

Description

Catalogue Number:SPK9142
Name::Human dsDNA(anti-double stranded DNA antibody IgG) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.096ng/mL
Standard: 20 ng/mL
Sample Type: serum, plasma, urine and other biological fluids
Assay Length: 3.5h
Research Area: disease
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to anti-double stranded DNA antibody IgG(dsDNA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to anti-double stranded DNA antibody IgG(dsDNA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain anti-double stranded DNA antibody IgG(dsDNA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of anti-double stranded DNA antibody IgG(dsDNA) in the samples is then determined by comparing the OD of the samples to the standard curve.