Human Dyn(Big Dynorphin) ELISA Kit

Description

Catalogue Number:SPK1675
Name::Human Dyn(Big Dynorphin) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 2.56 pg/mL
Standard: 500 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Signal transduction;Neuro science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Big Dynorphin(Dyn) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Big Dynorphin(Dyn). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Big Dynorphin(Dyn) in the samples is then determined by comparing the OD of the samples to the standard curve.