Human Histidyl-tRNA synthetase, cytoplasmic(HARS) ELISA kit

Description

Catalogue Number:SPK8405
Name::Human Histidyl-tRNA synthetase, cytoplasmic(HARS) ELISA kit
Species: Assay Type:Sandwich
Sensitivity: 5.4 pg/mL
Standard: 1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Biosynthesis/Metabolism
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HARS (Histidyl-tRNA synthetase, cytoplasmic). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HARS (Histidyl-tRNA synthetase, cytoplasmic). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HARS (Histidyl-tRNA synthetase, cytoplasmic), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HARS (Histidyl-tRNA synthetase, cytoplasmic) in the samples is then determined by comparing the OD of the samples to the standard curve.