Human HO1(Heme Oxygenase 1, Decycling) ELISA Kit

Description

Catalogue Number:SPK1476
Name::Human HO1(Heme Oxygenase 1, Decycling) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.119 ng/mL
Standard: 20 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heme Oxygenase 1, Decycling(HO1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heme Oxygenase 1, Decycling(HO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heme Oxygenase 1, Decycling(HO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heme Oxygenase 1, Decycling(HO1) in the samples is then determined by comparing the OD of the samples to the standard curve.