Human hs Ab1-42(high sensitivity Amyloid Beta Peptide 1-42) ELISA Kit

Description

Catalogue Number:SPK8920
Name::Human hs Ab1-42(high sensitivity Amyloid Beta Peptide 1-42) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 1.35 pg/mL
Standard: 300 pg/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Neuro science;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with high sensitivity Amyloid Beta Peptide 1-42(hs Ab1-42) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to high sensitivity Amyloid Beta Peptide 1-42(hs Ab1-42). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of high sensitivity Amyloid Beta Peptide 1-42(hs Ab1-42) in the samples is then determined by comparing the OD of the samples to the standard curve.