Human IL1e(Interleukin 1 Epsilon) ELISA Kit

Description

Catalogue Number:SPK4790
Name::Human IL1e(Interleukin 1 Epsilon) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 2.7 pg/mL
Standard: 500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Cytokine;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Epsilon(IL1e). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Epsilon(IL1e). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Epsilon(IL1e), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Epsilon(IL1e) in the samples is then determined by comparing the OD of the samples to the standard curve.