Human IL-37(Interleukin 37) ELISA Kit

Description

Catalogue Number:SPK1343
Name::Human IL-37(Interleukin 37) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 3.3 pg/mL
Standard: 500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 37(IL-37). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 37(IL-37). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 37(IL-37), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 37(IL-37) in the samples is then determined by comparing the OD of the samples to the standard curve.