Human KDM2B(Lysine (K) specific demethylase 2B) ELISA kit

Description

Catalogue Number:SPK9824
Name::Human KDM2B(Lysine (K) specific demethylase 2B) ELISA kit
Species: Assay Type:Sandwich
Sensitivity: 0.115 ng/mL
Standard: 20 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 3.5h
Research Area: Epigenetics and Nuclear Signaling
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysine (K) specific demethylase 2B(KDM2B). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lysine (K) specific demethylase 2B(KDM2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysine (K) specific demethylase 2B(KDM2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysine (K) specific demethylase 2B(KDM2B) in the samples is then determined by comparing the OD of the samples to the standard curve.