Syd Biotechnologies provides comprehensive protein purification services across multiple expression platforms, including bacterial, yeast, insect, and mammalian cell cultures. From gene design and codon optimisation to synthesis, cloning, expression, and purification, we offer an end-to-end solution tailored to your project needs. 

Our purification methods include gravity flow columns, low-pressure systems, and advanced Fast Protein Liquid Chromatography (FPLC) using Cytiva GE ÄKTA systems. Every protein is purified to high standards, with purity validated through SDS-PAGE and Size Exclusion Chromatography (SEC) profiling.  

Available Protein Purification Platforms  

Service Highlights 

• Complete, end-to-end solutions covering gene design, cloning, expression, and purification—tailored to your research needs. 

• Flexible tagging options including Fc, 6×His, GST, MBP, Strep(II), Protein A/G, Biotin, E-tag, and Calmodulin-binding peptide. 

• Purification strategies customised to your preference—whether affinity chromatography, ion-exchange, or size exclusion chromatography (SEC). 

• Rapid turnaround times:
  – 2–3 weeks for bacterial or yeast-expressed proteins
  – 3–6 weeks for mammalian systems
  – 6–8 weeks for insect expression platforms 

• Fully customised workflows with expert technical support at every stage. 

• Integrated downstream services including Immuno-flow cytometry, Circular Dichroism (CD), and NMR analysis—delivered from a single provider. 

Services Offered 

• Gene synthesis, cloning, and recombinant protein production and purification across various expression systems. 

• Antibody purification—both monoclonal and polyclonal—using high-quality Protein A/G columns from Pharmacia. 

• Tag or fusion protein removal from recombinant proteins using specific proteases. 

• Comprehensive protein characterisation through Western blotting, ELISA, Circular Dichroism (CD), SEC profiling, and flow cytometry. 

• Production of ¹³C- and ¹⁵N-labelled UltraPure proteins optimised for NMR spectroscopy studies. 

• Conjugation services to attach functional groups such as PEG, fluorophores, biotin, or streptavidin to proteins of interest. 

• Protein stability assays using Differential Loss in Fluorescence (DLF) and Circular Dichroism (CD) for biophysical evaluation. 

• Final processing solutions including salt exchange, desalting, concentration estimation, lyophilisation, and custom vialing. 

Custom Biophysical Assays 

Syd Biotechnologies offers customised biophysical assay services designed to support every stage of therapeutic development—from early discovery through validation and quality control. These assays are instrumental in progressing drug development by helping researchers: 

• Identify initial hits 

• Select lead candidates 

• Determine binding affinities 

• Analyse secondary and tertiary protein structures 

• Evaluate protein stability 

• Define overall protein conformation 

We provide high-quality protein preparations optimised for biophysical analysis, beginning from gene cloning to expression and purification using advanced FPLC and HPLC systems with affinity, size-exclusion (SEC), and ion-exchange (IEC) chromatography methods. 

UV Circular Dichroism Spectroscopy 

Syd Biotechnologies provides comprehensive custom Circular Dichroism (CD) spectroscopy services covering far-UV, near-UV, and visible wavelength ranges. Whether using our in-house FPLC/HPLC-purified samples or client-supplied materials, our experienced scientists meticulously optimize all parameters—including sample concentration, buffer composition, and cuvette path length—for precise and reliable CD analysis. 

Equipped with automated temperature control and titration systems, our CD spectroscopy setup ensures high-throughput and reproducible data acquisition. All analyses are conducted on the JASCO J-810 spectropolarimeter, which is routinely maintained and calibrated by certified JASCO engineers to ensure consistent performance and data accuracy. 

Service Highlights 

• Projects are handled by scientists trained in proteomics and spectroscopic studies.  

• Calibrated and maintained by JASCO periodically.  

• Appropriate controls are included.  

• Raw and analyzed data is provided at the end.  

• Rapid turnaround time.  

• Project customization.  

• One-stop service for gene cloning, expression, protein purification, CD, and NMR.  

Biacore Surface Plasmon Resonance Assay  

Syd Biotechnologies offers advanced Surface Plasmon Resonance (SPR) Biacore assay services, providing comprehensive support for assay development, target validation, high-throughput screening, and precise kinetic analysis. Whether you’re investigating protein–protein interactions or advancing hit-to-lead small molecule drug discovery, our SPR platform delivers accurate, real-time binding data to support your research and development goals effectively. 

Differential Scanning Fluorimetry (DSF) 

Syd Biotechnologies provides advanced Differential Scanning Fluorimetry (DSF) services to evaluate protein stability and uncover protein-ligand interactions. By analyzing thermal shifts in protein melting temperatures in the presence and absence of ligands, this technique helps identify potential binding compounds that enhance protein stability. Our skilled team utilizes cutting-edge instrumentation and analytical tools to deliver accurate, high-resolution data, supporting your drug discovery and protein engineering efforts with confidence. 

Nuclear Magnetic Resonance (NMR)  

Nuclear Magnetic Resonance (NMR) is a powerful analytical technique that exploits the behavior of certain nuclei—such as ¹H, ¹³C, ¹⁵N, and ¹⁹F—when subjected to a strong static magnetic field combined with a weak oscillating radiofrequency pulse. These NMR-active nuclei emit electromagnetic signals at characteristic frequencies influenced by their surrounding magnetic environment, allowing detailed molecular insights. 

NMR spectroscopy provides exceptionally sensitive information about atomic-level interactions, including chemical bonding, molecular conformation, folding states, and solvent accessibility of amino acid residues in proteins or nucleobases in RNA. This technique can be applied to samples in their natural isotopic composition or those enriched with specific NMR-active isotopes. 

Its ability to deliver high-resolution structural and dynamic information makes NMR an essential tool for studying biomolecules and small organic compounds across a wide range of chemical and biochemical research applications. 

Applications 

• Small Molecule CMC  

• Drug Discovery  

• Biosimilar Comparability  

• Protein Sequence, Structure, and Dynamics  

Custom Protein Lysate 

Syd Biotechnologies offers customized lysate preparation services designed to meet your unique research requirements. We specialize in generating high-quality lysates from a variety of sources, including human, mouse, and rat tissues, established cell lines, stem cells, membrane fractions, and overexpression systems. 

Our precisely prepared lysates are well-suited for numerous downstream applications such as SDS-PAGE, western blotting, ELISA, co-immunoprecipitation, pull-down assays, and studies of tissue-specific expression. These ready-to-use biological materials support a wide array of bioanalytical and pharmaceutical research workflows, providing reliable and reproducible results across diverse scientific disciplines. 

Service Highlights 

• Capable of processing tissue samples as small as a few milligrams with high efficiency. 

• Lysates can be prepared using either customized buffer formulations or standard commercial buffers, depending on project needs. 

• All buffers are supplemented with comprehensive phosphatase and protease inhibitor cocktails to preserve protein integrity. 

• Rigorous quality control is performed using SDS-PAGE followed by Coomassie Blue staining to assess protein profiles. 

• Final lysate concentrations are normalized to ensure consistency and accuracy across samples. 

These highlights reflect the robustness of our lysate preparation workflow—emphasizing flexibility, quality assurance, and precision to support reliable downstream applications.