Human MFI2(Melanotransferrin) ELISA Kit

Description

Catalogue Number:SPK3234
Name::Human MFI2(Melanotransferrin) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 12.9 pg/mL
Standard: 2000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanotransferrin(MFI2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Melanotransferrin(MFI2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Melanotransferrin(MFI2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Melanotransferrin(MFI2) in the samples is then determined by comparing the OD of the samples to the standard curve.