Human MVP(Major Vault Protein) ELISA Kit

Description

Catalogue Number:SPK4970
Name::Human MVP(Major Vault Protein) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.059 ng/mL
Standard: 10 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Vault Protein(MVP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Major Vault Protein(MVP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Vault Protein(MVP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Vault Protein(MVP) in the samples is then determined by comparing the OD of the samples to the standard curve.