Human N6AMT1(N-6-Adenine Specific DNA Methyltransferase 1) ELISA Kit

Description

Catalogue Number:SPK7564
Name::Human N6AMT1(N-6-Adenine Specific DNA Methyltransferase 1) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.264 ng/mL
Standard: 40 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-6-Adenine Specific DNA Methyltransferase 1(N6AMT1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-6-Adenine Specific DNA Methyltransferase 1(N6AMT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-6-Adenine Specific DNA Methyltransferase 1(N6AMT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-6-Adenine Specific DNA Methyltransferase 1(N6AMT1) in the samples is then determined by comparing the OD of the samples to the standard curve.