Custom Biophysical Assays 

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    Custom Biophysical Assays 

    Custom Biophysical Assays 

    Syd Biotechnologies offers customised biophysical assay services designed to support every stage of therapeutic development—from early discovery through validation and quality control. These assays are instrumental in progressing drug development by helping researchers: 

    • Identify initial hits 
    • Select lead candidates 
    • Determine binding affinities 
    • Analyse secondary and tertiary protein structures 
    • Evaluate protein stability 
    • Define overall protein conformation 

    We provide high-quality protein preparations optimised for biophysical analysis, beginning from gene cloning to expression and purification using advanced FPLC and HPLC systems with affinity, size-exclusion (SEC), and ion-exchange (IEC) chromatography methods. 

     

    UV Circular Dichroism Spectroscopy 

    Syd Biotechnologies provides comprehensive custom Circular Dichroism (CD) spectroscopy services covering far-UV, near-UV, and visible wavelength ranges. Whether using our in-house FPLC/HPLC-purified samples or client-supplied materials, our experienced scientists meticulously optimize all parameters—including sample concentration, buffer composition, and cuvette path length—for precise and reliable CD analysis. 

    Equipped with automated temperature control and titration systems, our CD spectroscopy setup ensures high-throughput and reproducible data acquisition. All analyses are conducted on the JASCO J-810 spectropolarimeter, which is routinely maintained and calibrated by certified JASCO engineers to ensure consistent performance and data accuracy. 

    Service Highlights 

    • Projects are handled by scientists trained in proteomics and spectroscopic studies.  
    • Calibrated and maintained by JASCO periodically.  
    • Appropriate controls are included.  
    • Raw and analyzed data is provided at the end.  
    • Rapid turnaround time.  
    • Project customization.  
    • One-stop service for gene cloning, expression, protein purification, CD, and NMR.  

    Biacore Surface Plasmon Resonance Assay  

    Syd Biotechnologies offers advanced Surface Plasmon Resonance (SPR) Biacore assay services, providing comprehensive support for assay development, target validation, high-throughput screening, and precise kinetic analysis. Whether you’re investigating protein–protein interactions or advancing hit-to-lead small molecule drug discovery, our SPR platform delivers accurate, real-time binding data to support your research and development goals effectively. 

    Differential Scanning Fluorimetry (DSF) 

    Syd Biotechnologies provides advanced Differential Scanning Fluorimetry (DSF) services to evaluate protein stability and uncover protein-ligand interactions. By analyzing thermal shifts in protein melting temperatures in the presence and absence of ligands, this technique helps identify potential binding compounds that enhance protein stability. Our skilled team utilizes cutting-edge instrumentation and analytical tools to deliver accurate, high-resolution data, supporting your drug discovery and protein engineering efforts with confidence. 

    Nuclear Magnetic Resonance (NMR)  

    Nuclear Magnetic Resonance (NMR) is a powerful analytical technique that exploits the behavior of certain nuclei—such as ¹H, ¹³C, ¹⁵N, and ¹⁹F—when subjected to a strong static magnetic field combined with a weak oscillating radiofrequency pulse. These NMR-active nuclei emit electromagnetic signals at characteristic frequencies influenced by their surrounding magnetic environment, allowing detailed molecular insights. 

    NMR spectroscopy provides exceptionally sensitive information about atomic-level interactions, including chemical bonding, molecular conformation, folding states, and solvent accessibility of amino acid residues in proteins or nucleobases in RNA. This technique can be applied to samples in their natural isotopic composition or those enriched with specific NMR-active isotopes. 

    Its ability to deliver high-resolution structural and dynamic information makes NMR an essential tool for studying biomolecules and small organic compounds across a wide range of chemical and biochemical research applications. 

    Applications 

    • Small Molecule CMC  
    • Drug Discovery  
    • Biosimilar Comparability  
    • Protein Sequence, Structure, and Dynamics  

     

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