Normalized cDNA Library
Syd Biotechnologies offers custom construction of normalized cDNA libraries designed to reduce the representation of high-abundance transcripts and enhance the detection of low-copy or rare genes. Depending on the client’s requirements, we apply one of several proven normalization strategies:
- Conversion of double-stranded cDNA from a standard library into single-stranded circular plasmids, followed by hybridization with complementary RNA (cRNA). The non-hybridized circular plasmids are then selectively converted back into double-stranded form to enrich underrepresented sequences.
- Normalization directly at the mRNA level prior to library synthesis, to equalize transcript abundance before cDNA synthesis.
- PCR-based normalization techniques, optimized according to the sample type and project objectives.
These methods help ensure a balanced and representative cDNA library, ideal for transcriptome analysis, gene discovery, and functional genomics research.
Service Highlights
- Normalized libraries with different Cot values (Cot 10, Cot 50, Cot 100)
- Normalization confirmed at various steps by northern, Q RT PCR, colony hybridization or sequencing
- Select the vector of your choice
- Both non-normalized (N0) and normalized (N1) libraries are delivered
- Receive two size fractionated libraries (0.4kb-2.0kb and 2.0 kb and up) for each sample
- 90-100% recombination efficiency
- >5 million primary clones supplied
- Phage resistant host competent cells used for propagation
- 250-500 µg endo-toxin free transfection grade DNA supplied with the library
- Semi-solid amplification procedure adapted for amplification that prevents biased clone representation
