Custom Phage Display Antibody Libraries
Syd Biotechnologies offers end-to-end construction of high-quality phage display antibody libraries from diverse species including mouse, human, rabbit, chicken, llama, and camelid. Our custom libraries are designed in scFv or Fab formats using advanced phagemid vectors, with variable light (vL) and heavy (vH) chains cloned either separately or together with flexible linker sequences. These libraries are ideal for screening and isolating high-affinity monoclonal antibodies for a wide range of applications.
Service Highlights
- Construction of scFv libraries with vL or vH chains individually in phagemid vectors
- Co-expression of recombinant vL and vH domains linked for scFv libraries
- Generation of Fab and (Fab’)₂ antibody libraries
- Development of nanobody (single-domain antibody) libraries from camelid and shark sources
- Creation of bispecific antibody libraries targeting two distinct antigens
- Custom design of bispecific ligand-antibody hybrids (one domain acts as an antibody, the other as a ligand)
- Specialized camelid (VHH) and shark (IgNAR) antibody library construction
Whether for therapeutic development, diagnostic innovation, or functional research, Syd Biotechnologies delivers tailor-made phage display libraries built for performance and scalability.
| Expression Strategies |
Purification Strategies |
- Secreted expression in periplasm of E. coli.
- Soluble expression in cytoplasm of E. coli.
- Insoluble expressed as inclusion bodies
- Secreted expression in 293 and CHO cells.
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- Affinity tag: 6xHis tag
- Molecular format: insertion of SUMO between His6 tag and protein of interest (POI)
- Purification: IMAC (Immobilized Metal Affinity Chromatography)
- Tag removal after purification: cleavage at the C terminus of SUMO with SUMO protease
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