Human PLCg1(Phospholipase C Gamma 1) ELISA Kit

Description

Catalogue Number:SPK2093
Name::Human PLCg1(Phospholipase C Gamma 1) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Enzyme & Kinase;Tumor immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase C Gamma 1(PLCg1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phospholipase C Gamma 1(PLCg1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase C Gamma 1(PLCg1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase C Gamma 1(PLCg1) in the samples is then determined by comparing the OD of the samples to the standard curve.