Human PSA(Puromycin Sensitive Aminopeptidase) ELISA Kit

Description

Catalogue Number:SPK4486
Name::Human PSA(Puromycin Sensitive Aminopeptidase) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.118 ng/mL
Standard: 20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Area: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Puromycin Sensitive Aminopeptidase(PSA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Puromycin Sensitive Aminopeptidase(PSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Puromycin Sensitive Aminopeptidase(PSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Puromycin Sensitive Aminopeptidase(PSA) in the samples is then determined by comparing the OD of the samples to the standard curve.