Human TRA2b(Transformer 2 Beta) ELISA Kit

Description

Catalogue Number:SPK7602
Name::Human TRA2b(Transformer 2 Beta) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.057 ng/mL
Standard: 10 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transformer 2 Beta(TRA2b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transformer 2 Beta(TRA2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transformer 2 Beta(TRA2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transformer 2 Beta(TRA2b) in the samples is then determined by comparing the OD of the samples to the standard curve.