Canine PGFM (Prostaglandin FM) ELISA Kit

Description

Catalogue Number:SPK0656
Name::Canine PGFM (Prostaglandin FM) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 3.3 pg/mL
Standard: 600 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area:
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PGFM. Standards or samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to PGFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. AfterTMB substrate solution is added, only those wells that contain PGFM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGFM in the samples is then determined by comparing the OD of the samples to the standard curve.