Mouse Activin-A(ACVA) ELISA Kit

Description

Catalogue Number:SPK8320
Name::Mouse Activin-A(ACVA) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 5 pg/mL
Standard: 600 pg/mL
Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Reproductive science;Genetic science;Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACVA(Activin-A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ACVA(Activin-A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ACVA(Activin-A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ACVA(Activin-A) in the samples is then determined by comparing the OD of the samples to the standard curve.