Mouse TGFb2(Transforming Growth Factor Beta 2) ELISA Kit

Description

Catalogue Number:SPK5216
Name::Mouse TGFb2(Transforming Growth Factor Beta 2) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 8.93 pg/mL
Standard: 2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
Assay Length: 2h
Research Area: Cytokine;Infection immunity;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Transforming Growth Factor Beta 2(TGFb2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 2(TGFb2). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta 2(TGFb2) in the samples is then determined by comparing the OD of the samples to the standard curve.