Rat Acly(ATP-citRate synthase) ELISA Kit

Description

Catalogue Number:SPK8567
Name::Rat Acly(ATP-citRate synthase) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.266 ng/mL
Standard: 40 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Metabonomics
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP-citRate synthase(Acly). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATP-citRate synthase(Acly). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP-citRate synthase(Acly), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP-citRate synthase(Acly) in the samples is then determined by comparing the OD of the samples to the standard curve.