Cattle VIP(Vasoactive Intestinal Peptide) ELISA Kit

Description

Catalogue Number:SPK7544
Name::Cattle VIP(Vasoactive Intestinal Peptide) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 2.63 pg/mL
Standard: 500 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Endocrinology;Gastroenterology;Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vasoactive Intestinal Peptide(Cattle VIP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vasoactive Intestinal Peptide(Cattle VIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vasoactive Intestinal Peptide(Cattle VIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vasoactive Intestinal Peptide(Cattle VIP) in the samples is then determined by comparing the OD of the samples to the standard curve.