Rat IgG1(Immunoglobulin G1) ELISA Kit

Description

Catalogue Number:SPK4760
Name::Rat IgG1(Immunoglobulin G1) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 18.7 ng/mL
Standard: 4000 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Area: Immune molecule;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin G1(IgG1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G1(IgG1). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G1(IgG1) in the samples is then determined by comparing the OD of the samples to the standard curve.