Rat Smad5(Mothers Against Decapentaplegic Homolog 5) ELISA Kit

Description

Catalogue Number:SPK8636
Name::Rat Smad5(Mothers Against Decapentaplegic Homolog 5) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 0.123 ng/mL
Standard: 20 ng/mL
Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Hematology;Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 5(Smad5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 5(Smad5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 5(Smad5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 5(Smad5) in the samples is then determined by comparing the OD of the samples to the standard curve.