Rat Total cholesterol (TC) ELISA Kit

Description

Catalogue Number:SPK8897
Name::Rat Total cholesterol (TC) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 2.63 μg/mL
Standard: 500 μg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Metabolic pathway;Hepatology;Gastroenterology;Nutrition metabolism;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TC(Total cholesterol ) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TC(Total cholesterol ). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TC(Total cholesterol ) in the samples is then determined by comparing the OD of the samples to the standard curve.