Rat TSLP(Thymic Stromal Lymphopoietin) ELISA Kit

Description

Catalogue Number:SPK6470
Name::Rat TSLP(Thymic Stromal Lymphopoietin) ELISA Kit
Species: Assay Type:Sandwich
Sensitivity: 5.7 pg/mL
Standard: 1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymic Stromal Lymphopoietin(TSLP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thymic Stromal Lymphopoietin(TSLP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymic Stromal Lymphopoietin(TSLP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymic Stromal Lymphopoietin(TSLP) in the samples is then determined by comparing the OD of the samples to the standard curve.