Sheep NEFA(Non-Esterified Fatty Acid) ELISA Kit

Description

Catalogue Number:SPK9685
Name::Sheep NEFA(Non-Esterified Fatty Acid) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 0.152 ng/mL
Standard: 20 ng/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 2h
Research Area: Kidney diseases
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Non-Esterified Fatty Acid(NEFA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Non-Esterified Fatty Acid(NEFA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Non-Esterified Fatty Acid(NEFA) in the samples is then determined by comparing the OD of the samples to the standard curve.