Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit

Description

Catalogue Number:SPK9768
Name::Dog HbA1c(Glycated Hemoglobin A1c) ELISA Kit
Species: Assay Type:Competitive Inhibition
Sensitivity: 4.25 μg/mL
Standard: 1000 μg/mL
Sample Type: plasma and erythrocyte lysates
Assay Length: 2h
Research Area: Diagnosis of diabetes mellitus
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glycated Hemoglobin A1c(HbA1c) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glycated Hemoglobin A1c(HbA1c). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycated Hemoglobin A1c(HbA1c) in the samples is then determined by comparing the OD of the samples to the standard curve.