Custom Circular RNA Synthesis
Syd Biotechnologies offers end-to-end circular RNA (circRNA) synthesis and analysis services, delivering high-quality results for advanced RNA research. Our workflow involves three key stages:
- Gene Cloning: The target circRNA-coding sequence is cloned into a suitable plasmid vector.
- In Vitro Transcription: Using T7 RNA polymerase, high-yield mRNA is transcribed and purified.
- Circularization: The linear mRNA is enzymatically ligated at both ends to form a circular RNA molecule. Any residual linear RNA is digested using RNase R to ensure purity.
The finalized circRNA is then further purified using urea polyacrylamide gel electrophoresis and thoroughly washed with RNase-free water, resulting in a clean, high-quality circRNA product ready for downstream applications.
Our Approach
To ensure the highest quality, the final circular RNA (circRNA) undergoes thorough validation, including absorbance readings at 260 and 280 nm, gel electrophoresis, and high-performance liquid chromatography (HPLC) profiling. Additionally, circular dichroism (CD) spectra are recorded for both linear and circular RNA to assess conformational differences.
Key Features of Our Service:
- Custom design of circRNAs with a variety of vector backbones suitable for efficient transfection and expression in eukaryotic cells.
- Functional evaluation of circRNA includes testing transcriptional efficiency and molecular stability in selected cell lines through in vitro assays.
This comprehensive approach ensures your circRNA constructs are optimized for both research and therapeutic applications.
Service Highlights
- Select the vector of your choice such as two-hybrid, retroviral or any other toxic vector
- Receive two size fractionated libraries (0.4kb-2.0kb and 2.0 kb and up) for each sample
- 90-100% recombination efficiency
- >5 million primary clones supplied
- Phage resistant host competent cells used for propagation
- 500 µg endo-toxin free DNA supplied with the library
- Semi-solid amplification procedure adapted for amplification preventing unbiased clone representation
