Custom PhIP-Seq Service for Profiling of Autoantibodies

Syd Biotechnologies offers Custom Phage ImmunoPrecipitation Sequencing (PhIP-Seq) services for comprehensive profiling of autoantibodies present in patient serum, leveraging advanced phage display technology.

This platform utilises a phage display library constructed from predesigned oligopeptides ranging from 50 to 90 amino acids, cloned into a modified T7 Select vector developed in-house at Syd Biotechnologies. The library is then screened using antibody pools isolated from patients with suspected or confirmed autoimmune disorders.

Following incubation, the antibody–phage complexes are selectively captured using Protein A/G resin. A two-step PCR process is employed: the first round amplifies and purifies the phage-insert sequences; the second round adds unique index barcodes to differentiate the samples. Sequencing is performed using the Illumina MiSeq platform.

Post-sequencing, FastQ files are demultiplexed, aligned, and analysed to determine enrichment patterns of patient-specific autoantibodies. PhIP-Seq is a powerful tool for investigating environmental exposures, allergies, emerging autoimmune conditions, and patient responses to immunomodulatory therapies.

Highlights:
·  Ready-to-Go modified T7 Select phage-display vector is available for immediate library construction.

• One-Stop CRO for designing, library construction, immunoprecipitation, and NGS services.
• Custom Vector modification project is accepted.
• Fast turnaround time with approximately 2-3 weeks for library construction, 1 week for immunoprecipitation and 1-2 weeks for NGS.
• Project customization and technical guidance are available throughout the project.
• Gene synthesis, cloning, and library construction.
• Screening of antibody pool by Immunoprecipitation using protein A/G resin.
• NGS and analysis.
• Validation Experiment.

Services Offered:
·  Gene synthesis, cloning, and library construction.

• Screening of antibody pool by Immunoprecipitation using protein A/G resin.
• NGS and analysis.
• Validation Experiment.