RNA Isolation

Syd Biotechnologies provides high-quality total RNA and mRNA isolation services from a diverse range of biological samples, including blood, animal tissues, plant materials, cultured cells, and other challenging specimens.

Our extraction process ensures RNA that is free from genomic DNA contamination (via DNase I treatment) and devoid of interfering substances like polysaccharides and phenolic compounds. RNA purity is verified through absorbance measurements at 260 nm and 280 nm, along with assessment on denaturing agarose gels.

The isolated RNA is of exceptional quality, making it ideal for a wide array of downstream applications such as microarray analysis, northern blotting, qRT-PCR, in vitro translation, RNase protection assays, full-length gene cloning, and cDNA library construction.

Service Highlights

• High quality total RNA and mRNA
• Free from genomic DNA and salts
• Quantitation by fluorometric assay for very low amount samples (pg or ng quantity)
• Additional services such as preparation of labeled probes for microarrays, first strand synthesis for QRTPCR, mRNA isolation are also offered
• First strand synthesis
• Dot blots, Norther Blots
• Cy-3 and Cy-5 labeling
• Synthesis of cRNA

DNA Isolation

We specialize in the generation of primary, immortalized, stable and knock-out cell lines. We have generated several cell lines for drug screening, diagnostics, protein expression and purification.

Transfection Grade Plasmid DNA

• Small as well as large scale isolation (micrograms to gram quantities)
• Purification by cesium chloride gradient or ion-exchange chromatography
• High throughput DNA isolation in 96-well format
• Ready for sequencing or PC
• Endotoxin level provided (if requested)

Viral DNA Isolation

• From infected E. coli or insect cell
• Purification over sucrose density gradient by ultra-centrifugation
• Extensively dialyzed in TE buffer
• C. on low percentage agarose gel and restriction enzyme digestion

Genomic DNA Isolation

• Un-sheared high molecular weight (>200 mega bases)
• Isolation from variety of tissues-(mammalian tissues, plants, bacteria, yeast and other challenging tissues rich in endogenous nucleases and phenolic compounds)
• Highly suitable for PCR, Southern Blot analysis, RAP
• D analysis, polymorphism studies and library construction