Phage Display:

Phage Display Surface Display  

Syd Biotechnologies offers premium custom phage display library construction and screening services to advance biological research and drug discovery initiatives. By introducing random mutations into the gene of interest, we generate a diverse array of peptides, small antibodies (such as scFv and Fab), or proteins, which are displayed as fusion proteins on the surface of filamentous phages (e.g., M13, fd, or f1 strains). 

Using a binding affinity-based method known as panning, phages that present proteins with specific affinity for a target molecule are selectively enriched from a library comprising billions of unique variants. The identities of these target-binding proteins are then determined through phage amplification followed by DNA sequencing. 

Our Approach  

Syd Biotechnologies employs the yeast display system to monitor antibody maturation. Antibodies expressed on the yeast cell surface are exposed to antigens under progressively stringent conditions. Yeast cells displaying strong antigen-binding are then isolated using Fluorescence-Activated Cell Sorting (FACS). The selected antibodies are subsequently purified using affinity chromatography. 

Service Highlights 

• Construction of phage display cDNA libraries tailored for specific research applications 
• Development of phage display peptide libraries with diversity ranging from 1×10⁸ to 1×10¹² variants 
• Library screening through multiple rounds (typically four) of panning using affinity chromatography and phage amplification 
• Screening of NEB’s Ph.D.™-7, Ph.D.™-12, and Ph.D.™-C7C peptide libraries 
• Screening and selection from custom phage display cDNA libraries 
• Bacterial expression and purification of scFv and Fab antibody fragments 
• Conversion of scFv and Fab fragments into humanised IgG antibodies, followed by expression in mammalian cell systems