Syd Biotechnologies specialises in the construction and screening of ribosome display libraries. These libraries, composed of diverse peptide sequences, cDNAs, and antibodies, are engineered with specific elements such as T7 RNA polymerase promoters, ribosome binding sites, actinomycin/puromycin interaction sites, and sequences to enhance RNA stability. Following in vitro transcription, translation is carried out using E. coli cell lysates, tRNA, buffer systems, and elevated Mg²⁺ concentrations at 4°C. Target–ligand–mRNA–ribosome quaternary complexes are then selectively captured and enriched through three to four iterative screening rounds. The RNA from these complexes is extracted, reverse transcribed, and cloned into a suitable vector. Finally, the resulting clones are sequenced and translated to determine the amino acid sequences of the selected ligands.
Schematic flow diagram showing various steps involved in a Ribosome Display experiment, including library synthesis, transcription, translation, target binding, washing, elution, and RNA isolation.
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